The resulting number of regions is reported in the The segmentation result is shown as a surface model Smoothing and grouping as specified in the (contour level) of the map should be adjustedīefore segmentation, because the calculation will use only the voxelsĬlicking Segment partitions the chosen map by the watershed method plus The map of interest should be chosen from the pulldown menu of open If interactive rotation speed is poor after segmentation, seeįrom the Volume Data section of the Tools menu So binning should aim for a grid spacing of approximately twice the Gaussian filtering reduces resolution to about six times the GaussianĪfter smoothing, it will also improve performance to reduce the sizeįor visualization and analysis, it is desirableįor a density map to be oversampled by three times in each dimension, Gaussian smoothing to reduce the number of local maxima It may be necessary to process a density map beforehandįor the segmentation to finish in a reasonable amount of time.Ī map of size 256 3 can have a million local maxima if it is noisy.
Own region, and the boundaries between regions lie at the Segment Map performs watershed segmentation:Ī density map is partitioned so that each local maximum has its Segmentation regions can be measured with Pintilie GD, Zhang J, Goddard TD, Chiu W, Gossard DC.Ĭomparison of Segger and other methods for segmentation and rigid-body docking of molecular components in cryo-EM density maps. Scale-space filtering, and fitting of structures by alignment to regions. Quantitative analysis of cryo-EM density map segmentation by watershed and
0 kommentar(er)
